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Phosphorylated MAP‐1B isoforms in the developing mouse barrel cortex
Author(s) -
Majewska Barbara,
SkangielKramska Jolanta
Publication year - 2000
Publication title -
international journal of developmental neuroscience
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.761
H-Index - 88
eISSN - 1873-474X
pISSN - 0736-5748
DOI - 10.1016/s0736-5748(99)00070-2
Subject(s) - immunocytochemistry , immunostaining , biology , phosphorylation , cortex (anatomy) , gene isoform , microbiology and biotechnology , barrel (horology) , synaptogenesis , barrel cortex , antibody , microtubule , neuroscience , immunohistochemistry , endocrinology , somatosensory system , biochemistry , immunology , gene , materials science , composite material
Developmental expression of two phosphorylation modes of microtubule‐associated protein 1B (MAP‐1B) has been studied in the barrel cortex of mice at postnatal days (P)5, P12, P21 and P90 using immunocytochemistry with antibodies 125 and 150 that recognize phosphorylation modes II and I, respectively. The antibody 125 immunoreactive processes, identified as dendrites, are not yet detectable at P5; they are already present at P12 and become more evident at P21. In the barrel cortex of P90 animals the antibody 125 immunopositive dendrites are still present, although they are much less pronounced. The antibody 150 punctate immunostaining seen at P5 is not detectable at P12. At P21, however, thin immunopositive fibres appear, implicating a re‐expression of the microtubule‐associated protein 1B phosphorylation mode I in a portion of axons. The antibody 150 immunopositive axons are no longer present in the P90 barrel cortex. The re‐expression of the MAP‐1B phosphorylation mode I, which is a juvenile isoform characteristic for growing axons, may imply induction of mechanisms providing mouse barrel cortex neurons with the potency for plastic changes at a terminal stage of synaptogenesis.