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Involvement of map kinase in the cyclic amp induction of myelin basic protein gene expression
Author(s) -
Clark Robert,
Stewart Mitchell,
Miskimins W.Keith
Publication year - 1998
Publication title -
international journal of developmental neuroscience
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.761
H-Index - 88
eISSN - 1873-474X
pISSN - 0736-5748
DOI - 10.1016/s0736-5748(98)00045-8
Subject(s) - myelin basic protein , myelin , oligodendrocyte , biology , microbiology and biotechnology , protein kinase a , mitogen activated protein kinase kinase , map kinase kinase kinase , c raf , kinase , ask1 , mapk14 , cellular differentiation , mitogen activated protein kinase , mapk7 , biochemistry , gene , neuroscience , central nervous system
Cyclic AMP is involved in the differentiation of oligodendrocyte and Schwann cell progenitors into mature myelin producing cells. The involvement of MAP kinases in this pathway was investigated in the D6P2T cell line. This cell line can be induced to display a differentiated phenotype characterized by myelin basic protein gene expression by increased cyclic AMP. BlockingMAPkinase activity with inhibitors of the activating kinase, MEK, by expression of a dominant negative MAP kinase or by expression of the MAPkinase inactivating phosphatase Mkp‐1 all blocked the activation of the myelin basic protein promoter in D6P2T cells. In addition, blocking MAP kinase activation during differentiation of an oligodendrocyte‐like cell line, CG4, also leads to inhibition of MBP expression. These findings suggest a role for MAP kinase in the cyclic AMP stimulated expression of the myelin basic protein gene during differentiation.