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Developmental regulation of mouse brain monomeric acetylcholinesterase
Author(s) -
Moreno Ricardo D.,
Campos Eliseo O.,
Dajas Federico,
Inestrosa Nibaldo C.
Publication year - 1998
Publication title -
international journal of developmental neuroscience
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.761
H-Index - 88
eISSN - 1873-474X
pISSN - 0736-5748
DOI - 10.1016/s0736-5748(98)00008-2
Subject(s) - acetylcholinesterase , aché , chemistry , embryonic stem cell , enzyme , monomer , embryo , biochemistry , microbiology and biotechnology , stereochemistry , biology , organic chemistry , gene , polymer
Acetylcholinesterase (AChE) molecular forms were studied during mouse brain development. Mouse embryos expressed a monomeric (G 1 ) and a tetrameric (G 4 ) AChE form. Our results indicate that G 4 AChE expressed at embryonic day (ED) 9 and ED15 could be purified by acridinium‐Sepharose chromatography and shared similar biochemical and kinetic properties with the adult form. However, the G 1 form expressed at either embryonic stage did not bind to acridinium, was not inhibited by excess substrate, and possessed higher K m and lower V max values than the adult G 1 form. Two peripheral anionic binding site inhibitors, fasciculin and propidium, had a significantly lower affinity for the monomeric form at ED9. Results are discussed in terms of the biological significance of the embryonic G 1 form, and its resemblance to the AChE activity found, associated with the senile plaques present in the brains of Alzheimer's patients.