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Developmental expression of nitric oxide synthase in the rat diencephalon with special reference to the thalamic paratenial nucleus
Author(s) -
Gorbatyuk Oleg,
Landry Marc,
Emson Piers,
Akmayev Ildar,
Hökfelt Tomas
Publication year - 1997
Publication title -
international journal of developmental neuroscience
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.761
H-Index - 88
eISSN - 1873-474X
pISSN - 0736-5748
DOI - 10.1016/s0736-5748(97)00045-2
Subject(s) - nucleus , diencephalon , nitric oxide synthase , in situ hybridization , hypothalamus , supraoptic nucleus , endocrinology , immunohistochemistry , arcuate nucleus , biology , thalamus , medicine , nitric oxide , chemistry , neuroscience , gene expression , biochemistry , gene
Using immunohistochemistry and in situ hybridization the distribution of nitric oxide synthase (NOS) was investigated in the rat brain during pre‐ and postnatal development. At E15 weak NOS‐like immunoreactivity (NOS‐LI) could be seen in the differentiation field of the anterior hypothalamus. At E17 strong NOS‐LI was observed in the developing neurons of the hypothalamic paraventricular nucleus, supraoptic nucleus, anterodorsal nucleus and lateral hypothalamic areas. In the thalamic paratenial nucleus a strong NOS‐LI was observed in these neurons at E17, E18 and P1 with a weaker intensity at P3, P7, P9 and P15, whereas at P30 and in adult rats no NOS‐positive neurons could be detected. NOS expression at E17 and P3 was verified by in situ hybridization. These results suggest that NO may have a developmental role at least in one of the regions studied, the thalamic paratenial nucleus.