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DNA polymerases δ and e in developing and aging rat brain
Author(s) -
Prapurna Danam Rebecca,
Rao Kalluri Subba
Publication year - 1997
Publication title -
international journal of developmental neuroscience
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.761
H-Index - 88
eISSN - 1873-474X
pISSN - 0736-5748
DOI - 10.1016/s0736-5748(96)00047-0
Subject(s) - polymerase , dna polymerase , dna , neuroscience , biology , psychology , microbiology and biotechnology , genetics
Our study reveals the presence of DNA polymerases δ and e , participating in DNA replication and repair, along with already known polymerases α and β , in the developing and aging rat brain. This was achieved through a protocol that takes advantage of the reported differential sensitivities of different DNA polymerases towards certain inhibitors such as butylphenyl and butylanilino nucleotide analogs, 2′, 3′‐dideoxythymidine triphosphate, the monoclonal antibody of human polymerase α and the use of preferred template primers and proliferating cell nuclear antigen. The results indicate that while polymerase β seems to be the predominant one, significant levels of polymerases α , δ and e are also present at all the postnatal ages studied and that the relative proportion of polymerase e increases with age. The data suggest that the rat brain is equipped with a sustained DNA repair capacity throughout the life span.