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Expression of fructose‐1,6‐bisphosphatase mRNA isoforms in normal and basal forebrain cholinergic lesioned rat brain
Author(s) -
Löffler Thomas,
AlRobaiy Samiya,
Bigl Marina,
Eschrich Klaus,
Schliebs Reinhard
Publication year - 2001
Publication title -
international journal of developmental neuroscience
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.761
H-Index - 88
eISSN - 1873-474X
pISSN - 0736-5748
DOI - 10.1016/s0736-5748(01)00011-9
Subject(s) - fructose 1,6 bisphosphatase , biology , in situ hybridization , gene isoform , messenger rna , astrocyte , biochemistry , microbiology and biotechnology , fructose , medicine , endocrinology , central nervous system , gene
Fructose‐1,6‐bisphosphatase is one of the key enzymes in the gluconeogenic pathway predominantly occurring in liver, kidney and muscle. In the brain, fructose‐1,6‐bisphosphatase has been suggested to be an astrocyte‐specific enzyme but the functional importance of glyconeogenesis in the brain is still unclear. To further elucidate the cellular source of fructose‐1,6‐bisphosphatase in the brain, non‐radioactive in situ hybridizations were performed using digoxigenin‐labeled RNA probes based on the sequence of recently cloned rat liver and muscle fructose‐1,6‐bisphosphatase cDNAs. In situ hybridization using a riboprobe for the liver isoform revealed a location of the hybridization signal mainly in neurons, while rat muscle fructose‐1,6‐bisphosphatase mRNA was detected in both neurons and astrocytes in the hippocampal formation and in layer I of the cerebral cortex. RT‐PCR using RNA preparations of rat astrocytes, neurons, and adult whole brain demonstrated a localization of liver fructose‐1,6‐bisphosphatase mRNA isoform in neurons but not in astrocytes. The muscle fructose‐1,6‐bisphosphatase mRNA isoform could be detected by RT‐PCR in total rat brain, astrocytic, and neuronal mRNA preparations. The isoforms of fructose‐1,6‐bisphosphatase mRNA seemingly demonstrate a distinct cellular expression pattern in rat brain suggesting a role of glyconeogenesis in both neurons and glial cells.