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Effects of transcutaneous topical injection of oxygen on vascular endothelial growth factor gene into the healing ligament in rats
Author(s) -
Ishii Yoshimasa,
Ushida Takashi,
Tateishi Tetsuya,
Miyanaga Yutaka
Publication year - 2003
Publication title -
journal of orthopaedic research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.041
H-Index - 155
eISSN - 1554-527X
pISSN - 0736-0266
DOI - 10.1016/s0736-0266(03)00056-1
Subject(s) - vascular endothelial growth factor , ligament , wound healing , subcutaneous injection , hindlimb , medicine , vegf receptors , oxygen , anatomy , chemistry , andrology , endocrinology , surgery , organic chemistry
The effects of intermittent exposure to oxygen injection on an experimentally induced ligament tear were studied in the right hind limb of 17 male Sprague–Dawley rats. Two rats were used for monitoring the partial oxygen pressure ( p O 2 ) of subcutaneous tissue and 15 rats were divided into the following three groups of 5 after an experimentally induced ligament tear: Group A, control group; Group B, injection of 0.5 ml hyaluronan to the wound transcutaneously; Group C, injection of 0.5 ml hyaluronan mixed with haemoglobin and oxygen ( n = 5). At 7 days post‐ligament injury, we compared the ligaments of the three treatment groups for gross appearance, histology and expression of vascular endothelial growth factor (VEGF) mRNA by RT‐PCR. Our results indicate that the p O 2 was immediately elevated to 334.6 mmHg by topical oxygen injection and this method was effective in promoting vessel formation in comparison to the control group ( p < 0.01). However, the expression of VEGF mRNA in the topical oxygen injection group (Group C) was lower than that in control group ( p < 0.05). Our results suggest that oxygen is able to accelerate vessel formation in spite of its effect of decreasing VEGF mRNA. Our method of using topical injection proved to be useful in healing the ligament and the wound. © 2003 Orthopaedic Research Society. Published by Elsevier Science Ltd. All rights reserved.