Premium
Bone induction by BMP‐2 transduced stem cells derived from human fat
Author(s) -
Dragoo Jason L.,
Choi Joon Y.,
Lieberman Jay R.,
Huang Jerry,
Zuk Patricia A.,
Zhang Jeffery,
Hedrick Marc H.,
Benhaim Prosper
Publication year - 2003
Publication title -
journal of orthopaedic research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.041
H-Index - 155
eISSN - 1554-527X
pISSN - 0736-0266
DOI - 10.1016/s0736-0266(02)00238-3
Subject(s) - bone morphogenetic protein , mesenchymal stem cell , microbiology and biotechnology , chemistry , bone morphogenetic protein 2 , bone marrow , bone morphogenetic protein 7 , extracellular matrix , biology , immunology , biochemistry , in vitro , gene
Purpose : We have isolated pluripotent mesenchymal progenitor cells in large numbers from liposuction aspirates (processed lipoaspirate cells or PLAs). This study examines the osteogenic potential of PLAs and bone marrow aspirate cells (BMAs), when exposed to either recombinant human bone morphogenetic protein (BMP)‐2 (rh‐ BMP‐2 ) or adenovirus containing BMP‐2 cDNA (Ad‐ BMP‐2 ). Methods : Liposuction aspirates underwent proteolytic digestion to obtain PLAs. After exposure to exogenous rh‐ BMP‐2 or Ad‐ BMP‐2 for four or seven days, PLAs and BMAs were assessed by histochemistry, spectrophotometry and RT‐PCR. Western blotting and ELISA confirmed BMP gene transduction. Results were compared to osteoblasts and cells in osteogenic media only. PLA‐Ad‐ BMP‐2 cells were seeded on matrices and implanted in the hind limbs of SCID mice. Results : Analysis of quantified bone precursor assays including extracellular ALP histomorphometry, intracellular ALP spectrophotometry, and calcified extracellular matrix (von Kossa) histomorphometry revealed that PLAs treated with exogenous rh‐ BMP‐2 or transduced with a BMP‐2 containing adenovirus (PLA‐Ad‐ BMP‐2 ) produced more bone precursors than osteoblasts ( p = 0.001). PLAs treated with exogenous rh‐ BMP‐2 or PLA‐Ad‐ BMP‐2 also produced more bone precursors than BMAs ( p = 0.001), except for day 7 ALP histomorphometry ( p = 0.343). ELISA confirmed successful BMP‐2 production by both progenitor cell groups transduced with Ad‐ BMP‐2 . H&E sections from collagen I matrices seeded with PLA‐Ad‐ BMP‐2 cells confirmed bone formation at six weeks. Conclusions : Liposuction aspirates contain PLAs that can be transfected with the BMP‐2 gene, with rapid induction into the osteoblast phenotype at a rate comparable to rh‐ BMP‐2 and osteoblast groups. Transduced PLAs produce more bone precursors with faster onset of calcified extracellular matrix than transduced BMAs. PLAs may be an ideal source of mesenchyme‐lineage stem cells for gene therapy and tissue engineering. © 2003 Orthopaedic Research Society. Published by Elsevier Science Ltd. All rights reserved.