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Engineered allogeneic mesenchymal stem cells repair femoral segmental defect in rats
Author(s) -
Tsuchida Hiroyuki,
Hashimoto Junichi,
Crawford Eric,
Manske Paul,
Lou Jueren
Publication year - 2003
Publication title -
journal of orthopaedic research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.041
H-Index - 155
eISSN - 1554-527X
pISSN - 0736-0266
DOI - 10.1016/s0736-0266(02)00108-0
Subject(s) - mesenchymal stem cell , progenitor cell , bone healing , bone morphogenetic protein , bone marrow , osteoblast , stem cell , bone morphogenetic protein 2 , stem cell transplantation for articular cartilage repair , genetic enhancement , microbiology and biotechnology , medicine , cancer research , immunology , pathology , biology , adult stem cell , cellular differentiation , anatomy , gene , in vitro , genetics
Bone marrow derived mesenchymal stem cells (MSC) have been shown to be progenitor cells for mesenchymal tissues. These cells may also provide a potential therapy for bone repair. Our previous studies showed that MSC engineered with the gene for bone morphogenetic protein 2 (BMP‐2), a growth factor for bone cells, were capable of differentiating into osteoblast lineage and inducing autologous bone formation in several animal models. Culturing individual MSC for autologous implantation, however, remains problematic. The number of human MSC with osteogenic potential decreases with age, and, in certain diseases, the patient's marrow may be damaged or the healthy cells reduced in number. In this study, we used rats with a femoral segmental defect to investigate whether allogeneic BMP‐2 engineered MSC would facilitate bone healing. We show that BMP‐2 engineered allogeneic MSC can repair critical bone defects to the same degree as rats treated with BMP‐2 engineered autologous MSC, if the allogeneic group receives short‐term treatment with immunosuppressant FK506. We also show that allogeneic gene transferred MSC are directly involved in bone repair, in addition to acting as gene deliverers. © 2002 Orthopaedic Research Society. Published by Elsevier Science Ltd. All rights reserved.