Premium
Transplanted bone marrow cells localize to fracture callus in a mouse model
Author(s) -
Devine Michael J.,
Mierisch Cay M.,
Jang Euichan,
Anderson Peggy C.,
Balian Gary
Publication year - 2002
Publication title -
journal of orthopaedic research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.041
H-Index - 155
eISSN - 1554-527X
pISSN - 0736-0266
DOI - 10.1016/s0736-0266(02)00051-7
Subject(s) - callus , bone marrow , stromal cell , bone healing , in vivo , bone fracture , pathology , biology , chemistry , medicine , anatomy , botany , microbiology and biotechnology , radiology
Bone marrow contains many cellular elements that may contribute to fracture repair. We used a pluripotential stromal cell in a mouse model to demonstrate the presence of transplanted cells in fracture hematoma and subsequently in maturing fracture callus. Cells were transduced with traceable genes (lac Z and neomycin resistance) and traced in vivo after intravenous injection into syngeneic mice. These transduced cells home to bone marrow, suggesting that they might be detected in fracture callus. Cells were injected intravenously into mice and stabilized femoral shaft fractures were induced. Control mice received intravenous lactated‐Ringer's solution prior to fracture. Callus tissue and marrow were examined histologically from 1 to 10 weeks after fracture to detect transplanted cells. Transplanted cells were detected in fracture callus in areas, and at times, of most active bone formation. Control specimens showed minimal staining of the callus tissue. Levels of the traceable gene in fracture callus increased, reached a peak between 3 and 4 weeks after fracture, then diminished and disappeared by 10 weeks post‐fracture as woven bone at the fracture site was replaced by lamellar bone with cells from the host mouse. The results show that pluripotent bone marrow cells home to the marrow after systemic injection and localize in fracture callus. © 2002 Orthopaedic Research Society. Published by Elsevier Science Ltd. All rights reserved.