Premium
Clonality studies in giant cell tumor of bone
Author(s) -
Schwartz H. S.,
Eskew J. D.,
Butler M. G.
Publication year - 2002
Publication title -
journal of orthopaedic research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.041
H-Index - 155
eISSN - 1554-527X
pISSN - 0736-0266
DOI - 10.1016/s0736-0266(01)00117-6
Subject(s) - loss of heterozygosity , biology , giant cell tumor of bone , telomere , locus (genetics) , carcinogenesis , microsatellite , microbiology and biotechnology , genetics , cancer research , allele , giant cell , dna , cancer , gene
Genetic studies including chromosome analysis, telomere reduction and telomere activity, DNA microsatellites and loss of heterozygosity (LOH) studies have been performed on giant cell tumor (GCT) of bone however whether this primary skeletal neoplasm represents a monoclonal or polyclonal proliferation is unknown. Utilizing a new assay to study the polymorphic human androgen receptor locus (HUMARA), the ratio of maternal inactive X‐chromosome to the paternal inactive X (Lyon hypothesis) is determined via a methylation – specific polymerase chain reaction (PCR) technique to detect X‐chromosome polymorphisms. Characterization of the genetic tumorigenesis of this unpredictable neoplasm may lend insight into its biological behavior and offer improvements in therapeutic intervention, as new information emerges regarding osteoclastic bone resorption. Seventeen female patients with giant cell tumor of bone had their DNA harvested and their X‐chromosome inactivation pattern and polymorphisms determined and compared to control. A polyclonal proliferation pattern was identified in all informative samples studied. © 2002 Orthopaedic Research Society. Published by Elsevier Science Ltd. All rights reserved.