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Differential expression of neural cell adhesion molecule (NCAM) after tenotomy in rabbit skeletal muscle
Author(s) -
Jamali Amir A.,
Afshar Pouya,
Abrams Reid A.,
Lieber Richard L.
Publication year - 2002
Publication title -
journal of orthopaedic research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.041
H-Index - 155
eISSN - 1554-527X
pISSN - 0736-0266
DOI - 10.1016/s0736-0266(01)00095-x
Subject(s) - neural cell adhesion molecule , tenotomy , cell adhesion molecule , rabbit (cipher) , microbiology and biotechnology , skeletal muscle , l1 , cell adhesion , adhesion , neuroscience , chemistry , cell , biology , anatomy , computer science , biochemistry , tendon , gene , computer security , organic chemistry
Tenotomy is a commonly encountered event in orthopaedic surgery. In 23 rabbit extensor digitorum longus (EDL) muscles, within 24 h after tenotomy, a marked drop in maximum force production occurred. This was not explainable based on architectural changes and histological examination using standard markers for muscle injury, i.e., haematoxylin and eosin morphology, developmental myosin heavy chain (MHC) immunolabeling, and quantitation of muscle fiber type percentage, area and distribution. The expression of neural cell adhesion molecule (NCAM), a glycoprotein expressed during muscle development was measured as a function of time in these muscles. NCAM expression was increased as early as one day after tenotomy with 2.2 ± 1.2% of the fibers showing positive expression. This expression level increased significantly to 15.4 ± 15.2% after 7 days and then subsided to 13.2 ± 10.6% 21 days after tenotomy. Two‐way analysis of variance demonstrated a significant effect of time and a significant time x tenotomy method interaction. These results suggest that tenotomy leads to possible changes in muscle‐nerve connections and/or excitation–contraction (EC) coupling. © 2002 Orthopaedic Research Society. Published by Elsevier Science Ltd. All rights reserved.