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BMP‐12 gene transfer augmentation of lacerated tendon repair
Author(s) -
Lou Jueren,
Tu Yizheng,
Burns Meghan,
Silva Matthew. J.,
Manske Paul
Publication year - 2001
Publication title -
journal of orthopaedic research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.041
H-Index - 155
eISSN - 1554-527X
pISSN - 0736-0266
DOI - 10.1016/s0736-0266(01)00042-0
Subject(s) - tendon , gene transfer , bone morphogenetic protein , bone morphogenetic protein 2 , microbiology and biotechnology , progenitor cell , tendon transfer , genetic enhancement , chemistry , mesenchymal stem cell , in vivo , anatomy , in vitro , gene , biology , stem cell , genetics , biochemistry
Bone morphogenetic protein (BMP) 12 is a recently discovered member of the human BMP family. It is the human homolog of mouse growth/differentiation factor (GDF)‐7. Previously we reported that injection of mesenchymal progenitor cells transferred with the BMP‐12 gene into the muscles of nude mice induced tendon‐like tissue formation. In this study, we further investigated the effect of BMP‐12 gene transfer on tendon cells. We observed that adenovirus mediated in vitro BMP‐12 gene transfer into chicken tendon cells increased type I collagen synthesis. No change in alkaline phosphatase activity was observed following BMP‐12 gene transfer. We also determined that BMP‐12 gene transfer into a complete tendon laceration chicken model resulted in a two‐fold increase of tensile strength and stiffness of repaired tendons, indicating improved tendon healing in vivo. We conclude that BMP‐12 gene transfer is a promising procedure for improving the tendon repair process. © 2001 Orthopaedic Research Society. Published by Elsevier Science Ltd. All rights reserved.