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Gamma irradiation of HIV‐1
Author(s) -
Smith Richard A.,
Ingels Jesse,
Lochemes John J.,
Dutkowsky Joseph P.,
Pifer Linda L.
Publication year - 2001
Publication title -
journal of orthopaedic research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.041
H-Index - 155
eISSN - 1554-527X
pISSN - 0736-0266
DOI - 10.1016/s0736-0266(01)00018-3
Subject(s) - sterilization (economics) , gamma irradiation , virus , reverse transcriptase , in vitro , human immunodeficiency virus (hiv) , virology , immunofluorescence , syncytium , irradiation , cell culture , biology , antibody , immunology , polymerase chain reaction , biochemistry , physics , genetics , monetary economics , foreign exchange market , foreign exchange , gene , nuclear physics , economics
The potential for transmission of human immunodeficiency virus (HIV) type 1 has created serious concern for the continued clinical use of bone and soft‐tissue allografts. Tissue banks have employed 1.5–2.5 Mrad for sterilization of bone and tendon allografts, which, according to the current literature, approaches the level at which the tissue quality is adversely affected for implantation. Our working hypothesis was that gamma irradiation at increasing doses can proportionately inactivate HIV type 1. The objective of this study was to inactivate HIV type 1 by irradiation, as determined by its capacity to infect human T‐lymphocytes and established cell lines in vitro. The replicative competence of HIV type 1 was also assessed by the presence of reverse transcriptase activity, enzyme‐linked immunoadsorbent assay (ELISA), immunofluorescence assays for p24 viral core antigen, and the formation of syncytia induced by HIV type 1 in the cultures inoculated with irradiated virus. The results demonstrated the presence of active viral replication in previously noninfected cells in the supernatant samples that were exposed to as much as 5.0 Mrad. The data for the 10‐Mrad sample were indeterminate due to cellular damage. These data suggest that gamma irradiation (1.5–2.5 Mrad) does not constitute a virucidal dose for HIV type 1. Current technologies for screening have greatly improved, and the surgeon should rely on tissue bank screening procedures and other methods of preparation rather than sterilization by gamma radiation techniques in choosing allograft material. © 2001 Orthopaedic Research Society. Punlished by Elsevier Science Ltd. All rights reserved.