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The effects of reduced oxygen tension on cell proliferation and matrix synthesis in synovium and tendon explants from the rabbit carpal tunnel: an experimental study in vitro
Author(s) -
Rempel David,
Abrahamsson SvenOlof
Publication year - 2001
Publication title -
journal of orthopaedic research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.041
H-Index - 155
eISSN - 1554-527X
pISSN - 0736-0266
DOI - 10.1016/s0736-0266(00)00005-x
Subject(s) - tendon , hypoxia (environmental) , oxygen tension , chemistry , cell growth , anatomy , synovial membrane , extracellular matrix , matrix (chemical analysis) , explant culture , microbiology and biotechnology , in vitro , medicine , immunology , biology , oxygen , biochemistry , inflammation , organic chemistry , chromatography
Local ischemia may play an important role in the development of tendon degeneration as well as entrapment neuropathies. In order to investigate the cellular effects of hypoxia on tendon and synovial tissue from the carpal canal, dose response effects of oxygen on cell proliferation and synthesis of matrix components were examined in segments of synovial and flexor digitorum profundus tendon from the carpal tunnel of rabbits during short term culture. Explants were incubated in airtight containers flushed with either 0%, 1%, 3%, 20% O 2 plus 2% CO 2 and N 2 to balance and labeled with either 3 H‐thymidine or 3 H‐proline and 35 S‐sulfate. Cell proliferation was significantly inhibited by hypoxia in synovium but not in tendon ( P = 0.03). In parallel, the synthesis of non‐collagenous proteins was significantly reduced in synovium but not in tendon ( P = 0.006). In both tissues hypoxia significantly inhibited collagen synthesis. On the other hand, hypoxia had no significant effect on the synthesis of new proteoglycans as determined by 35 S‐sulfate incorporation. Hypoxia can inhibit cell proliferation and alter synthesis of matrix components in synovial tissue, but may only have minor effects on non‐collagen protein synthesis in tendon explants from the carpal canal of rabbit forepaws. © 2001 Orthopaedic Research Society. Published by Elsevier Science Ltd. All rights reserved.