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The three subtypes of atrial natriuretic peptide (ANP) receptors are expressed in the rat adrenal gland
Author(s) -
Grandclément Béatrice,
Ronsin Brice,
Morel Gérard
Publication year - 1997
Publication title -
biology of the cell
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.543
H-Index - 85
eISSN - 1768-322X
pISSN - 0248-4900
DOI - 10.1016/s0248-4900(99)80079-3
Subject(s) - zona glomerulosa , adrenal gland , biology , medicine , endocrinology , receptor , in situ hybridization , aldosterone , atrial natriuretic peptide , adrenal cortex , messenger rna , pinealocyte , microbiology and biotechnology , pineal gland , angiotensin ii , gene , biochemistry , melatonin , blood pressure
Atrial natriuretic peptide (ANP) actions are mediated by highly selective and specific receptors. Three subtypes have been characterized and cloned: ANP receptor‐A (or GC‐A), ‐B (or GC‐B) and ‐C (the so‐called clearance receptor). In rat adrenal gland, the mRNA for each subtype was detected using 35 S‐dUTP or digoxigenin‐11‐dUTP specific labeled probes, and in situ hybridization at light and electron microscopic levels respectively. The three subtypes were expressed the most abundantly in the zona glomerulosa. The amount of GC‐A mRNA expression, quantified using macroautoradiography and densitometry, was higher than the amounts of GC‐B mRNA and ANPR‐C mRNA both in zona glomerulosa and medullary of adrenal gland. At electron microscopic level, the three subtypes of ANPR were revealed in glomerulosa cells. A noticeable signal was also present in the medullary area, especially for GC‐A mRNA, in adrenaline‐containing chromaffin cells. No signal was detected in noradrenaline‐containing chromaffin cells. The subcellular localization of the three mRNAs is similar: in the cytoplasmic matrix and in the euchromatin of the nucleus in each cell of glomerulosa, and in the same compartments of the adrenaline‐containing chromaffin cells. These data indicate that the adrenal gland is an important target tissue for ANP action both in glomerulosa cells and adrenaline‐containing chromaffin cells. The mRNA expression levels were different for each ANPR subtype.