Premium
Glycoconjugate and adhesion molecule changes during the cell cycle in a human embryonic epithelial cell line
Author(s) -
Fantin Anna Maria Bolognani,
Franchini Antonella,
Malgara Roberta,
Rebecchi Barbara,
Conti Anna Maria Fuhrman
Publication year - 1998
Publication title -
biology of the cell
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.543
H-Index - 85
eISSN - 1768-322X
pISSN - 0248-4900
DOI - 10.1016/s0248-4900(98)80336-5
Subject(s) - biology , glycoconjugate , cytoplasm , microbiology and biotechnology , laminin , mitosis , fibronectin , cell adhesion molecule , cell cycle , cell adhesion , integrin , proteoglycan , cell culture , cell , extracellular matrix , biochemistry , genetics
The changes in the expression of glycoconjugates and adhesion molecules were studied by selective lectin binding and immunocytochemical reactions in a human embryonic epithelial cell line (EUE cells), synchronized in the cell cycle phases. The results can be summarized as follows: most of the tested lectins display a more diffuse binding for the cytoplasm in G 1 than S and G 2 phases; in the S and particularly in G 2 phases the cytoplasm glycoconjugates are rearranged around the nucleus; cells in mitosis always show a strong binding towards all tested lectins. Cellular fibronectin and its receptor β 1 integrin are well expressed in G 1 , but the strongest reaction is observed in the S phase. The immunoreactions for laminin and uvomorulin (L‐CAM) are poorly positive in all cell cycle phases. The immunocytochemical reaction for heparan sulfate is positive, with a stronger reaction in S and G 2 than in G 1 ; on the contrary a diffuse staining with the anti‐dermatan sulfate proteoglycan antibody appears unchanged during the cell cycle.