Premium
A simple technique for the long‐term non‐polarised and polarised culture of human fallopian tube epithelial cells
Author(s) -
Kervancioglu M.E.,
Saridogan E.,
Martin J.E.,
Maguiness S.D.,
Djahanbakhch O.
Publication year - 1994
Publication title -
biology of the cell
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.543
H-Index - 85
eISSN - 1768-322X
pISSN - 0248-4900
DOI - 10.1016/s0248-4900(94)80012-x
Subject(s) - fallopian tube , biology , cytokeratin , cell culture , epithelium , extracellular matrix , immunofluorescence , in vitro , anatomy , matrigel , staining , microbiology and biotechnology , pathology , andrology , immunohistochemistry , antibody , immunology , medicine , biochemistry , genetics
Summary— Fallopian tubes were obtained from 25 women undergoing abdominal hysterectomy. Pieces of fallopian tube mucosa were placed in culture flasks containing minimum essential medium in Earle's salts supplemented with fetal bovine serum. First passage was carried out after 7–10 days and subcultures in 4–5 days. For polarised cell culture, epithelial cells were seeded onto an extracellular matrix system. New epithelial cells were seen on day 2–3 of the primary culture and epithelial patches on day 7–10. Cells reached confluence in 4–5 days in subcultures. The cells could be subcultured for 7–11 passages with a life span of 42–60 days. Epithelial origins of the cells were confirmed by immunofluorescence staining with anti‐cytokeratin antibody. Polarised cells showed a columnar pattern, microvilli on their apical surface and basally located nucleus whereas non‐polarised cells were flat. It was concluded that the human fallopian tube epithelial cells can be cultured in vitro to create non‐polarised and polarised cell layers by using a simple and reproducible technique and this system can be a potential model to study function of the fallopian tube.