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Effects of suramin, a polyanionic drug inducing lysosomal storage disorders on tooth germs in vitro
Author(s) -
GritliLinde Amel,
Ruch JeanVictor,
Mark Manuel P.,
Lécolle Sylvie,
Goldberg Michel
Publication year - 1994
Publication title -
biology of the cell
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.543
H-Index - 85
eISSN - 1768-322X
pISSN - 0248-4900
DOI - 10.1016/s0248-4900(94)80005-7
Subject(s) - suramin , ameloblast , biology , dentinogenesis , basement membrane , odontoblast , microbiology and biotechnology , melanosome , vacuole , in vitro , biochemistry , pharmacology , cytoplasm , dentin , pathology , enamel paint , medicine , melanin , dentistry
Summary— Suramin, a potent inhibitor of lysosomal enzymes, is commonly employed as a tool for inducing experimental mucopolysaccharidosis and lipidosis. The effects of the drug on embryonic mouse molars were analysed. Presecretory ameloblasts and odontoblasts were loaded with lysosome‐like vacuoles. Staining with MC22‐33F, an antibody to choline phospholipids and sphingomyelin, was completely reversed in the suramin‐treated germs, in that it stained only presecretory ameloblasts ( versus odontoblasts and some pulpal cells in the control group), according to a developmentally regulated pattern. The suramin‐induced cytoplasmic changes were reminiscent of the features of mucopolysaccharidoses and lipidoses. The basement membrane, separating the enamel organ from the dental papilla, displayed suramin‐induced patches, and in predentin collagen fibrillogenesis was found to be disturbed. Furthermore, autoradiography was employed to reveal uptake and distribution of [ 3 H] suramin in the cells and predentin. Finally, a suramin‐induced disturbance of the metabolism of sulphated macromolecules was found. The results imply that suramin effects in vitro on tooth germs can be used as a useful experimental model with to study both the action of the drug as well as cell and extracellular matrix perturbations in a mucopolysaccharidosis‐like condition.