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Immunodetection of low concentrations of ovalbumin in cryoprocessed quail oviduct cells by semi‐automatic quantitative analysis
Author(s) -
Quintana Carmen,
LópezIglesias Carmen,
Bonnet Noel,
Lebonvallet Stephane
Publication year - 1993
Publication title -
biology of the cell
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.543
H-Index - 85
eISSN - 1768-322X
pISSN - 0248-4900
DOI - 10.1016/s0248-4900(05)80202-3
Subject(s) - immunolabeling , quail , biology , ovalbumin , oviduct , immunogold labelling , cytoplasm , microbiology and biotechnology , ultrastructure , anatomy , immunology , antigen , endocrinology , immunohistochemistry
Summary— In a previous paper (Quintana et al, Biol Cell 72, (1991) 167–180) we reported the anti‐ovalbumin antibody immunolabeling in the cytoplasm of tubular gland cells from cryofixed, cryosubstituted and acrylic‐resin embedded quail oviduct. To confirm these preliminary visual observations, we have carried out a semi‐automatic quantitative study of immunogold labeling. The quantitative results confirm the previous data. In addition, we found a significantly higher immunolabeling with low temperature Lowicryl K11M embedding procedure as compared with high temperature LR White embedding.