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Stimulation of peroxisomal palmitoyl‐CoA oxidase activity by ciprofibrate in hepatic cell lines: Comparative studies in Fao, MH 1 C 1 and HepG2 cells
Author(s) -
Brocard Cécile,
EsSouni Malika,
Ramirez Leyla C,
Latruffe Norbert,
Bournot Paulette
Publication year - 1993
Publication title -
biology of the cell
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.543
H-Index - 85
eISSN - 1768-322X
pISSN - 0248-4900
DOI - 10.1016/s0248-4900(05)80172-8
Subject(s) - peroxisome , stimulation , oxidase test , biology , cell culture , clofibric acid , enzyme , biochemistry , cell , microbody , microbiology and biotechnology , endocrinology , medicine , gene , genetics
Summary— The response of two rat cell lines, Fao and MH 1 C 1 , and one human cell line, HepG2, to the peroxisome proliferator ciprofibrate, was studied. Using a fluorometric assay for palmitoyl‐CoA oxidase, the dose‐ and time‐dependent increase of this enzymatic activity was determined. From the lowest concentration (100 μM) stimulation is evident in the two rat cell lines. In the Fao line, the activity was stimulated reaching a seven‐fold increase over the control level at 250 μM after 72 h of treatment. In the MH 1 C 1 line, the maximum stimulation, four‐ to five‐fold, was obtained at 250 and 500 μM after 72 h. In the HepG2 cell line, activity increased two‐fold at 250 μM after 72 h reaching a three‐fold increase at 1000 μM after 48 h. Ciprofibrate was more toxic to Fao cells than to MH 1 C 1 and HepG2 cells which is also the order of the acyl‐CoA oxidase stimulation by ciprofibrate. These preliminary results suggest that the two rat cell lines are appropriate for investigating the induction of peroxisomal β‐oxidation enzymes and the expression of their genes. The HepG2 cell line is a complementary model for the study of interspecies differences in the response to peroxisomal proliferators and of the peroxisomal functions implied in the lipid metabolism of human liver.