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New type of snRNP containing nuclear bodies in plant cells
Author(s) -
Niedojadło Janusz,
GórskaBrylass Alicja
Publication year - 2003
Publication title -
biology of the cell
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.543
H-Index - 85
eISSN - 1768-322X
pISSN - 0248-4900
DOI - 10.1016/s0248-4900(03)00061-3
Subject(s) - snrnp , biology , cajal body , fibrillarin , rna splicing , microspore , microbiology and biotechnology , small nuclear rna , spirogyra , tenera , nucleolus , cytoplasm , genetics , rna , botany , stamen , gene , pollen , agroforestry , rna dependent rna polymerase , palm oil , algae
In larch ( Larix decidua Mill.) microspores a new type of nuclear bodies has been found which are an element of the spatial organization of the splicing system in plant cell. These are bizonal bodies, ultrastructurally differentiated into a coiled part and a dense part. Using immunocytochemistry and in situ hybridization at the EM level, the coiled part of the bizonal body was found to contain snRNA including U2 snRNA, Sm proteins and nucleolar proteins of the agyrophilic type and fibrillarin. The dense part contains Sm proteins but lacks snRNA. Such a separation of macromolecules related to splicing occurring within the bizonal bodies microspore is striking by the similarity of these bodies to amphibian oocyte snurposomes. The occurrence in plant cells, beside widely known coiled bodies (CBs), also of other nuclear bodies related to splicing proves that in plants similarly as for animals the differentiation among domains containing elements of the splicing system occurs.