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Evolutionary conservancy of the endocytic and trafficking machinery in the unicellular eukaryote Paramecium
Author(s) -
Surmacz Liliana,
Wiejak Jolanta,
Wyroba Elzbieta
Publication year - 2003
Publication title -
biology of the cell
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.543
H-Index - 85
eISSN - 1768-322X
pISSN - 0248-4900
DOI - 10.1016/s0248-4900(03)00012-1
Subject(s) - biology , endocytic cycle , eukaryote , paramecium , dynamin , endosome , microbiology and biotechnology , homology (biology) , endocytosis , gene , genetics , genome , cell , intracellular
Molecular search for the homologues of the mammalian proteins in the unicellular eukaryote Paramecium involved in endocytosis and membrane trafficking is discussed. We cloned and sequenced the gene fragments encoding the following components participating in endosome formation, sorting and maturation of the proprotein precursors, respectively, dynamin 2, Rab7 and furin. There is a proof that all these genes are expressed in this unicellular organism. The function of the identified immunoanalogues of the above described components of Paramecium endocytic machinery as well as a high degree of sequence homology to the respective human counterparts points to the evolutionary conservancy of these pathways.