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Permeability changes induced by polylysines in rat spermatids
Author(s) -
Jorquera Ramon A.,
Berrios Julio,
Sans Jorge,
Vergara Cecilia,
Benos Dale J.,
Reyes Juan G.
Publication year - 2002
Publication title -
biology of the cell
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.543
H-Index - 85
eISSN - 1768-322X
pISSN - 0248-4900
DOI - 10.1016/s0248-4900(02)01198-x
Subject(s) - biology , intracellular , calcein , lysis , divalent , quenching (fluorescence) , biophysics , spermatid , membrane , nigericin , biochemistry , permeability (electromagnetism) , ethidium bromide , cytosol , membrane permeability , fluorescence , microbiology and biotechnology , chemistry , dna , endocrinology , spermatogenesis , enzyme , physics , organic chemistry , quantum mechanics
High molecular weight (HMW, >15 kDa) but not low molecular weight (LMW, <15 kDa) polylysines (PLs) bound and induced permeability changes in rat spermatid plasma membranes, estimated by Mn 2+ quenching of intracellular indo‐1 fluorescence ( K 1/2 = 3.3 ± 0.5 μg/ml) and Co 2+ quenching of intracellular calcein. The pharmacology of the Mn 2+ entry pathway activated by HMW PL does not suggest that Ca 2+ channels are involved in this phenomenon. Concentrations of HMW PL that induced divalent ion entry did not induce the entry of ethidium bromide, suggesting that HMW PL first bound and perturbed the plasma membrane structure inducing a non‐specific increase in membrane permeability. High concentrations of HMW PL induced cell lysis ( K 1/2 = 23 μg/ml). The binding of HMW PL, initially homogenous on the cell surface, subsequently progressed to a segregated pattern resembling a clustering phenomenon.