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MAP kinase dynamics in yeast
Author(s) -
Drogen Frank,
Peter Matthias
Publication year - 2001
Publication title -
biology of the cell
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.543
H-Index - 85
eISSN - 1768-322X
pISSN - 0248-4900
DOI - 10.1016/s0248-4900(01)01123-6
Subject(s) - fluorescence recovery after photobleaching , biology , kinase , microbiology and biotechnology , subcellular localization , mitogen activated protein kinase , cytoplasm , scaffold protein , nuclear localization sequence , photobleaching , signal transduction , biochemistry , fluorescence , physics , quantum mechanics , membrane
Summry— MAP kinase pathways play key roles in cellular responses towards extracellular signals. In several cases, the three core kinases interact with a scaffold molecule, but the function of these scaffolds is poorly understood. They have been proposed to contribute to signal specificity, signal amplification, or subcellular localization of MAP kinases. Several MAP kinases translocate to the nucleus in response to their activation, suggesting that nuclear transport may provide a regulatory mechanism. Here we describe new applications for Fluorescence Recovery After Photobleaching (FRAP) and Fluorescence Loss In Photobleaching (FLIP), to study dynamic translocations of MAPKs between different subcellular compartments. We have used these methods to measure the nuclear/cytoplasmic dynamics of several yeast MAP kinases, and in particular to address the role of scaffold proteins for MAP‐kinase signaling.