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Intracellular pH in one‐cell mouse embryo differs between subcellular compartments and between interphase and mitosis
Author(s) -
Amirand Claudine,
Mentré Pascale,
Geijn Sjoerd,
Waksmundzka Malgorzata,
Debey Pascale
Publication year - 2000
Publication title -
biology of the cell
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.543
H-Index - 85
eISSN - 1768-322X
pISSN - 0248-4900
DOI - 10.1016/s0248-4900(00)01080-7
Subject(s) - interphase , biology , mitosis , nucleolus , cytoplasm , microbiology and biotechnology , chromatin , pronucleus , cell cycle , embryo , nucleoplasm , intracellular , transcription (linguistics) , cell , zygote , genetics , embryogenesis , dna , linguistics , philosophy
The pH‐sensitive dual‐emission fluorophore SNARF‐1 coupled with a laser confocal microspectrofluorimeter was used to measure the internal pH (pH i ) in different subcellular and subnuclear compartments of early mouse embryos. By this method we analysed the first cell cycle of naturally fertilised embryos in order to detect possible pH i changes correlated to cellular events, particularly the onset of replication or transcription and the first mitosis. Throughout interphase, significant differences of pH i were observed between cytoplasm and pronuclei, and, even more striking, between these compartments and nucleolus precursor bodies, whose pH i was systematically lower. We could detect significant pH i change neither during the replication phase nor at the onset of zygotic transcription, but pH i increased at the end of the one‐cell stage in both cytoplasm and chromatin regions, a process that seemed specifically correlated with mitosis.