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Total free radical trapping antioxidant potential in pre‐eclampsia
Author(s) -
Kharb S.
Publication year - 2000
Publication title -
international journal of gynecology and obstetrics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.895
H-Index - 97
eISSN - 1879-3479
pISSN - 0020-7292
DOI - 10.1016/s0020-7292(99)00198-8
Subject(s) - lipid peroxidation , uric acid , medicine , antioxidant , eclampsia , vitamin e , vitamin c , radical , superoxide , ascorbic acid , endocrinology , biochemistry , oxidative stress , chemistry , food science , pregnancy , enzyme , biology , genetics
Objective : The aim of the present study was to measure serum urate, tocopherol and ascorbate and to calculate total radical trapping antioxidant potential (TRAP) in pre‐eclampsia. Methods : Study samples were taken from 25 pre‐eclamptic, 25 normotensive pregnant and 25 non‐pregnant healthy females for measuring uric acid, vitamin C and E, total sulfhydryl group. Also lipid peroxidation product malonaldehyde (MDA), the superoxide generation (NBT reduction) were estimated and TRAP was calculated. Data were analyzed statistically using Student's t ‐test. Result : Pre‐eclamptic women had significantly increased MDA levels, superoxide generation and uric acid levels as compared to control subjects ( P <0.001). Vitamin C and E and total thiols were lower in pre‐eclampsia as compared to control subjects ( P <0.001). TRAP was significantly higher in pre‐eclampsia. Conclusion : TRAP provides a means of assessing the total capacity of the chain‐breaking antioxidants to prevent lipid peroxidation in plasma.