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Detection of human papillomavirus types in cervical adenocarcinoma by the polymerase chain reaction
Author(s) -
Lee M.F.,
Chang M.C.,
Wu C.H.
Publication year - 1998
Publication title -
international journal of gynecology and obstetrics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.895
H-Index - 97
eISSN - 1879-3479
pISSN - 0020-7292
DOI - 10.1016/s0020-7292(98)00171-4
Subject(s) - polymerase chain reaction , medicine , human papillomavirus , adenocarcinoma , cervix , pathogenesis , restriction enzyme , virology , dna , uterine cervix , pathology , gene , carcinoma , cancer , biology , genetics
To determine the human papillomavirus (HPV) types in cervical adenocarcinoma of patients from Taiwan. Methods: DNA was extracted from fixed tissues and polymerase chain reaction was performed in conjunction with a unique probe, pRSA I, allowing simultaneous detection of HPV types 6, 11, 16, 18, 31 and 33 from amplified HPV DNAs after endonuclease, Rsa I, digestion. Results: Of 69 tissues examined, 31.9% (22/69) were found to contain HPV DNA. Among 22 HPV‐positive specimens, no HPV types 6, 11, 31 and 33 were detected. On the other hand, HPV 16 and HPV 18 were found in 11 (15.9%) and 10 (14.5%) of HPV‐positive specimens, respectively. One specimen (1.5%) was found to contain both HPV 16 and 18 DNAs. Conclusions: Our findings support that HPV 18, along with HPV 16, may play a certain role in the adenocarcinoma pathogenesis of the uterine cervix.