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Isolation and characterisation of the human rab18 gene after stimulation of endothelial cells with histamine
Author(s) -
Schäfer Ute,
Seibold Stefan,
Schneider Annette,
Neugebauer Edmund
Publication year - 2000
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(99)01778-0
Subject(s) - umbilical vein , northern blot , complementary dna , biology , microbiology and biotechnology , histamine , signal transduction , gene expression , rab , gene , gtpase , biochemistry , endocrinology , in vitro
Here we report the isolation of a cDNA encoding the complete human rab18 protein from histamine‐stimulated endothelial cells using differential display. The amino acid sequence showed 98% homology with the previously isolated mouse rab18 protein, which is implicated in apical/basolateral endocytosis. Northern blot analysis revealed two transcripts (2.5 kb and 1 kb) ubiquitously expressed in all examined organs, as well as in human umbilical vein endothelial and aortic cells. In blood cells rab18 transcripts were hardly detectable. The histamine‐induced time‐dependent increase of rab18 mRNA expression in human umbilical vein endothelial cells and peripheral blood mononuclear cells indicates for the first time a link between receptor‐mediated signal transduction and the regulation of rab gene expression. This finding might also imply a role for rab proteins in inflammation.