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Mildly oxidised low density lipoprotein induces platelet shape change via Rho‐kinase‐dependent phosphorylation of myosin light chain and moesin
Author(s) -
Retzer Michaela,
Siess Wolfgang,
Essler Markus
Publication year - 2000
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(99)01762-7
Subject(s) - myosin light chain kinase , phosphorylation , myosin , moesin , rho associated protein kinase , chemistry , microbiology and biotechnology , kinase , thienopyridine , platelet activation , platelet , biochemistry , biophysics , cytoskeleton , medicine , biology , clopidogrel , ezrin , aspirin , cell
Oxidised low density lipoprotein (LDL) plays an important role in the pathogenesis of atherosclerosis. Here we demonstrate that mildly oxidised (mox) LDL engages the GTPase Rho and its effector molecule p160 Rho‐kinase to induce phosphorylation of myosin light chain and of moesin leading to platelet shape change. Pretreatment of platelets with the selective Rho inhibitor C3‐transferase from Clostridium botulinum or with the Rho‐kinase inhibitor Y‐27632 blocked mox‐LDL‐induced myosin light chain phosphorylation, moesin phosphorylation and shape change. Mox‐LDL did not induce an increase in cytosolic Ca 2+ during shape change. We propose that Rho/Rho‐kinase inhibition could be a strategy for prevention of the pathologic platelet activation during atherogenesis.