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Molecular cloning and functional expression of Ca v 3.1c, a T‐type calcium channel from human brain
Author(s) -
Cribbs Leanne L.,
Gomora Juan Carlos,
Daud Asif N.,
Lee Jung-Ha,
Perez-Reyes Edward
Publication year - 2000
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(99)01756-1
Subject(s) - genbank , intron , alternative splicing , complementary dna , exon , cloning (programming) , gene , genetics , biology , gene isoform , molecular cloning , microbiology and biotechnology , transfection , calcium channel , calcium , chemistry , computer science , programming language , organic chemistry
Low voltage‐activated T‐type calcium channels are encoded by a family of at least three genes, with additional diversity created by alternative splicing. This study describes the cloning of the human brain α1G, which is a novel isoform, Ca v 3.1c. Comparison of this sequence to genomic sequences deposited in the GenBank allowed us to identify the intron/exon boundaries of the human CACNA1G gene. A full‐length cDNA was constructed, then used to generate a stably‐transfected mammalian cell line. The resulting currents were analyzed for their voltage‐ and time‐dependent properties. These properties identify this gene as encoding a T‐type Ca 2+ channel.