Premium
Inositol 1,4,5‐trisphosphate receptor associated with focal contact cytoskeletal proteins
Author(s) -
Sugiyama Tomoyasu,
Matsuda Yuzuru,
Mikoshiba Katsuhiko
Publication year - 2000
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(99)01732-9
Subject(s) - vinculin , cytoskeleton , paxillin , inositol , microbiology and biotechnology , signal transducing adaptor protein , receptor , actin , cytoplasm , focal adhesion , chemistry , actin cytoskeleton , biology , signal transduction , biochemistry , cell
The linkage between inositol 1,4,5‐trisphosphate (IP 3 ) receptors (IP 3 Rs) and cytoskeletal proteins is considered to be important in cell function. In the present study, the association of IP 3 R subtypes with cytoskeletal proteins was examined using monoclonal antibodies specific to each IP 3 R subtype. We found that IP 3 R type 2 colocalized with talin, a focal contact cytoskeletal protein. IP 3 R type 2 exhibited a patchy distribution in the peripheral cytoplasm differently from type 1 and type 3 IP 3 R. Furthermore, IP 3 R subtypes co‐immunoprecipitated with talin, vinculin and α‐actin, but not α‐actinin or paxillin.