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A kinetic study of the oxidation effects of amphotericin B on human low‐density lipoproteins
Author(s) -
Barwicz Joanna,
Gruda Ilona,
Tancrède Pierre
Publication year - 2000
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(99)01731-7
Subject(s) - amphotericin b , chemistry , oxidizing agent , low density lipoprotein , oxidative phosphorylation , lipoprotein , high density lipoprotein , biochemistry , toxicity , pharmacology , cholesterol , antifungal , organic chemistry , microbiology and biotechnology , medicine , biology
The UV‐visible results of this kinetic study show that amphothericin B as Fungizone is a much stronger oxidant than CuSO 4 , itself a powerful oxidant of low‐density lipoprotein (LDL). Amphotericin B as AmBisome alone has no oxidizing effect on LDL while a mixture of both AmBisome and CuSO 4 induces an important potentialization of the LDL oxidation. These results allow us to believe that the high toxicity of amphotericin B is related to its capacity to modify and to weaken the structure of LDL. In addition, differential scanning calorimetry experiments show that the oxidative modifications of LDL by CuSO 4 or by amphotericin B proceed through different mechanisms.