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Inverse distribution of uncoupling proteins expression and oxidative capacity in mature adipocytes and stromal‐vascular fractions of rat white and brown adipose tissues
Author(s) -
Prunet-Marcassus Bénédicte,
Moulin Karine,
Carmona Maria Carmen,
Villarroya Francesc,
Pénicaud Luc,
Casteilla Louis
Publication year - 1999
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(99)01710-x
Subject(s) - ucp3 , thermogenin , oxidative phosphorylation , uncoupling protein , stromal vascular fraction , brown adipose tissue , white adipose tissue , adipose tissue , endocrinology , chemistry , medicine , prdm16 , oxidative stress , biology , biochemistry
To investigate relationships between the uncoupling protein (UCP) family and oxidative metabolism in fat pads, we measured the cytochrome oxidase activity, used as an index of oxidative capacity, and the mRNA content encoding UCP1, UCP2 and UCP3. Most oxidative potential was found in the stromal‐vascular fraction (SVF) of brown fat and in mature adipocytes of white fat (inguinal and periovarian). Considering the whole fat pads, the oxidative potential observed in mature white adipocytes fraction was not negligible compared with that of brown adipocytes fraction. UCP1 and UCP3 were expressed exclusively in mature brown adipocytes. Whatever the deposit, UCP2 mRNA was mainly localized in the SVF. These results indicate that, in fat, high oxidative potential is not necessarily linked to high UCPs transcripts content and point out the oxidative capacity of SVF from brown fat.