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The role of phosphatidylinositide‐3‐kinase in basal mitogen‐activated protein kinase activity and cell survival
Author(s) -
Versteeg Henri H,
Evertzen Maaike W.A,
van Deventer Sander J.H,
Peppelenbosch Maikel P
Publication year - 2000
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(99)01701-9
Subject(s) - mitogen activated protein kinase kinase , mapk/erk pathway , protein kinase a , map kinase kinase kinase , cyclin dependent kinase 2 , microbiology and biotechnology , cyclin dependent kinase 9 , ask1 , map2k7 , c raf , kinase , mitogen activated protein kinase , chemistry , biology
Phosphatidylinositide‐3‐OH‐kinase (PI 3‐kinase) is an upstream activator of p42/p44 mitogen‐activated protein kinase (MAPK), but the role of PI 3‐kinase‐dependent MAPK remains obscure. Here we demonstrate that in a variety of different cell types, PI 3‐kinase inhibition results in an inhibition of MAPK in unstimulated cells but does not interfere with growth factor‐, or TPA‐induced MAPK activity. Furthermore, inhibition of either PI 3‐kinase or MEK/MAPK results in cell death in serum‐starved cells. We concluded that basal, but not induced MAPK activity is mediated by PI 3‐kinase and that this PI 3‐kinase‐mediated MEK/MAPK activity is essential for cell survival in quiescent cells.