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Prediction of human cDNA from its homologous mouse full‐length cDNA and human shotgun database
Author(s) -
Fukunishi Yoshifumi,
Suzuki Harukazu,
Yoshino Masayasu,
Konno Hideaki,
Hayashizaki Yoshihide
Publication year - 1999
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(99)01696-8
Subject(s) - complementary dna , human genome , homology (biology) , shotgun sequencing , coding region , biology , exon , genetics , genome , shotgun , intron , gene , microbiology and biotechnology , computational biology
We propose a prediction method for human full‐length cDNA by comparing sequence data between human genome shotgun sequence and mouse full‐length cDNA. The human genome which is homologous to the mouse full‐length cDNA is selected by a homology search program, and the predicted exons are connected at the exon‐intron junction which gives the best homology score to the mouse full‐length cDNA. The accuracy of the predicted human full‐length coding region is 83.3%, and the false positive rate is 16.7%. Five human full‐length proteins out of 20 proteins are correctly predicted.