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The tRNA N 2, N 2‐dimethylguanosine‐26 methyltransferase encoded by gene trm1 increases efficiency of suppression of an ochre codon in Schizosaccharomyces pombe
Author(s) -
Niederberger Christian,
Gräub Remo,
Costa Annie,
Desgrès Jean,
Schweingruber M.Ernst
Publication year - 1999
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(99)01679-8
Subject(s) - schizosaccharomyces pombe , transfer rna , gene , genetics , codon usage bias , schizosaccharomyces , biology , start codon , rna , messenger rna , saccharomyces cerevisiae , genome
In the majority of eukaryotic tRNAs, the guanosine at position 26 is modified by a dimethyl group, but so far a function of this modification has not been detected. We isolated the Schizosaccharomyces pombe gene, trm1 , encoding the tRNA N 2, N 2‐dimethylguanosine‐26 methyltransferase. Strains having the gene deleted completely lack N 2, N 2‐dimethylguanosine. In strains carrying the weak ochre tRNA suppressor sup3 ‐i, deletion of trm1 abolishes suppression indicating that the trm1 deletion acts as an antisuppressor mutation. The result suggests that in vivo N 2, N 2‐dimethylguanosine‐26 increases the capacity of the sup3 ‐i serine tRNA to translate the UAA ( ochre ) codon.