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Different induction of gulonolactone oxidase in aromatic hydrocarbon‐responsive or ‐unresponsive mouse strains
Author(s) -
Braun László,
Kardon Tamás,
Koulali Karim El,
Csala Miklós,
Mandl József,
Bánhegyi Gábor
Publication year - 1999
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(99)01649-x
Subject(s) - in vivo , chemistry , oxidase test , signal transduction , transcription (linguistics) , biochemistry , enzyme inducer , enzyme , microbiology and biotechnology , biology , linguistics , philosophy
The role of aromatic hydrocarbon receptor (AhR)‐mediated signal transduction pathways was investigated in the regulation of ascorbate synthesis by using Ah‐responsive and Ah‐unresponsive mouse strains. In vivo 3‐methylcholanthrene treatment increased hepatic and plasma ascorbate concentrations only in the Ah‐responsive strain. The mRNA level of gulonolactone oxidase and the microsomal ascorbate production from p ‐nitrophenyl glucuronide, D ‐glucuronic acid or gulonolactone in the liver of Ah‐responsive and Ah‐unresponsive mice were compared. In Ah‐responsive mice, these parameters were higher originally, and they further increased upon in vivo addition of 3‐methylcholanthrene, while in Ah‐unresponsive mice the treatment was not effective. These results suggest that the transcription of gulonolactone oxidase gene is regulated by an Ah receptor‐dependent signal transduction pathway.