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(−)‐Epigallocatechin gallate inhibits Mos activation‐mediated Xenopus oocyte maturation induced by progesterone
Author(s) -
Yasokawa Masayo,
Sugimoto Isamu,
Fukuma Motoyo,
Goto Natsuko,
Kobayashi Naoki,
Yasuda Hiroko,
Ogura Kumiko,
Iwashita Jun,
Nagata Katsumi,
Hiji Yasutake,
Hashimoto Eikichi
Publication year - 1999
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(99)01646-4
Subject(s) - microinjection , xenopus , oocyte , protein kinase a , chemistry , phosphorylation , western blot , maturation promoting factor , gallate , protein kinase c , microbiology and biotechnology , epigallocatechin gallate , biology , biochemistry , polyphenol , apoptosis , cyclin dependent kinase 1 , embryo , cell cycle , nuclear chemistry , gene , antioxidant
(−)‐Epigallocatechin gallate (EGCG), one of the constituents of green tea known to have a tumor preventing effect, inhibited maturation of Xenopus laevis oocytes induced by progesterone when this polyphenol was microinjected into oocytes at a final concentration of about 1 mM. Western blot and activity measurement analyses showed that Mos translation and the subsequent activations of mitogen‐activated protein kinase and p90 rsk , probably by protein phosphorylation, seemed to have been inhibited by the microinjection of EGCG. These results suggest that EGCG may have the ability to control Xenopus oocyte maturation at least during the stage of Mos activation.