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Glucose degradation product methylglyoxal enhances the production of vascular endothelial growth factor in peritoneal cells: role in the functional and morphological alterations of peritoneal membranes in peritoneal dialysis
Author(s) -
Inagi Reiko,
Miyata Toshio,
Yamamoto Takashi,
Suzuki Daisuke,
Urakami Ken-ichi,
Saito Akira,
van Ypersele de Strihou Charles,
Kurokawa Kiyoshi
Publication year - 1999
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(99)01642-7
Subject(s) - methylglyoxal , vascular endothelial growth factor , peritoneal dialysis , peritoneum , angiogenesis , vascular permeability , endocrinology , growth factor , vascular endothelial growth factor a , medicine , peritoneal equilibration test , chemistry , biochemistry , continuous ambulatory peritoneal dialysis , pathology , vegf receptors , receptor , enzyme
Peritoneal membrane permeability deteriorates in peritoneal dialysis (PD) patients. We test whether glucose degradation products (GDPs) in PD fluids, glyoxal, methylglyoxal and 3‐deoxyglucosone, stimulate the production of vascular endothelial growth factor (VEGF), a factor known to enhance vascular permeability and angiogenesis. VEGF increased in cultured rat mesothelial and human endothelial cells exposed to methylglyoxal, but not to glyoxal or 3‐deoxyglucosone. VEGF also increased in peritoneal tissue of rats given intraperitoneally methylglyoxal. VEGF and carboxymethyllysine (CML) (formed from GDPs) co‐localized immunohistochemically in mesothelial layer and vascular walls of the peritoneal membrane of patients given chronic PD. By contrast, in the peritoneum of non‐uremic subjects, VEGF was identified only in vascular walls, in the absence of CML. VEGF production induced by GDPs may play a role in the progressive deterioration of the peritoneal membrane.