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Simultaneous core 2 β1→6 N ‐acetylglucosaminyltransferase up‐regulation and sialyl‐Le X expression during activation of human tonsillar B lymphocytes
Author(s) -
Nakamura Mitsuru,
Ishida Takashi,
Kikuchi Jiro,
Furukawa Yusuke,
Matsuda Michio
Publication year - 1999
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(99)01619-1
Subject(s) - core (optical fiber) , microbiology and biotechnology , chemistry , biology , physics , optics
We have investigated the regulation mechanism of the surface sialyl‐Le X (sLe X ) expression level in tonsillar B cells during activation. sLe X antigen became strongly positive after activation, while resting B cells were weakly positive. sLe X structures were mainly located on O ‐linked oligosaccharide chains of glycoprotein. Transcripts of FucT‐VII and core 2 GlcNAc transferase (C2GnT) were up‐regulated after activation, while those of ST3GalIV and β1→4GalT‐I were expressed constitutively. However, the up‐regulation of C2GnT was more dramatic than that of FucT‐VII. These results suggest that sLe X expression level is regulated by C2GnT during tonsillar B cell activation.