Premium
The T3 b gene promoter directs intestinal epithelial cell‐specific expression in transgenic mice
Author(s) -
Aihara Hiroyuki,
Hiwatashi Nobuo,
Kumagai Shinji,
Obata Yuhichi,
Shimosegawa Tooru,
Toyota Takayoshi,
Miyazaki Jun-ichi
Publication year - 1999
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(99)01608-7
Subject(s) - transgene , biology , microbiology and biotechnology , genetically modified mouse , promoter , gene , gene expression , genetics
Although a few promoters that direct intestinal epithelial cell‐specific expression in transgenic animals have been reported, they are not necessarily appropriate for transgenic studies in terms of activity and tissue specificity. Here, we examined the tissue specificity of transgene expression directed by the 2.8‐kb promoter region of the T3 b gene, which encodes one of the non‐classical major histocompatibility complex class I molecules. The transgene was expressed exclusively in the epithelial cells of the small and large intestines at high levels. The results indicate that the T3 b promoter is useful for directing transgene expression specifically in intestinal epithelial cells.