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Hypertrophic stimuli augment expression of cMG1/ERF‐1, a putative zinc‐finger motif transcription factor, in rat cardiomyocytes
Author(s) -
Manabe Tomohiro,
Fukuda Keiichi,
Pan Jing,
Nagasaki Koichi,
Yamaguchi Ken,
Ogawa Satoshi
Publication year - 1999
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(99)01601-4
Subject(s) - wortmannin , zinc finger , chemistry , microbiology and biotechnology , transcription factor , endothelin 1 , messenger rna , biology , gene , signal transduction , protein kinase b , biochemistry , receptor
We isolated the gene for cMG1/ERF‐1, a known putative zinc‐finger transcription factor, by differential display of mRNA extracted from cardiomyocytes with and without leukemia inhibitory factor (LIF) stimulation. LIF induced cMG1/ERF‐1 mRNA at 15 min, and levels peaked at 10‐fold initial levels at 30 min. cMG1/ERF‐1 expression was inhibited by AG490 (JAK2 inhibitor) and genistein, but was unaffected by PD98059 or wortmannin. Phenylephrine, angiotensin II and endothelin‐1 also induced cMG1/ERF‐1 expression. Mechanical stretch in vitro and acute pressure overload in vivo increased cMG1/ERF‐1 expression. To our knowledge, this is the first report showing that the cMG1/ERF‐1 gene can be induced by various hypertrophic stimuli, and that Janus kinase 2 is involved in this process.