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Cloning of bovine RANTES mRNA and its expression and regulation in ovaries in the periovulatory period
Author(s) -
Aust Gabriela,
Brylla Elke,
Lehmann Irina,
Kiessling Silke,
Spanel-Borowski Katharina
Publication year - 1999
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(99)01599-9
Subject(s) - messenger rna , in situ hybridization , cloning (programming) , follicle , theca , microbiology and biotechnology , biology , ovarian follicle , ovary , andrology , endocrinology , gene , biochemistry , medicine , computer science , programming language
RANTES may be one of the chemoattractants involved in stimulating eosinophils and macrophages to migrate selectively into bovine dominant follicles and into developing corpora lutea. We sequenced a 736 bp fragment of the bovine RANTES mRNA encoding the complete protein and defined the ovarian source of RANTES mRNA. As demonstrated by competitive RT‐PCR, follicle‐derived macrophages showed a 100–1000 times higher RANTES mRNA level compared to unpurified granulosa cells or follicle‐derived fibroblasts. By means of in situ hybridization, RANTES mRNA positive macrophages were located in the former thecal layer of the developing corpora lutea.