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Inhibitory effect of Pax4 on the human insulin and islet amyloid polypeptide (IAPP) promoters
Author(s) -
Campbell Susan C.,
Cragg Helen,
Elrick Lucy J.,
Macfarlane Wendy M.,
Shennan Kathleen I.J.,
Docherty Kevin
Publication year - 1999
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(99)01584-7
Subject(s) - pax4 , insulinoma , peptide sequence , islet , biology , promoter , microbiology and biotechnology , chemistry , biochemistry , insulin , transcription factor , gene , pancreas , endocrinology , homeobox , gene expression
Pax4 is a paired‐box transcription factor that plays an important role in the development of pancreatic β‐cells. Two Pax4 cDNAs were isolated from a rat insulinoma library. One contained the full‐length sequence of Pax4. The other, termed Pax4c, was identical to Pax4 but lacked the sequences encoding 117 amino acids at the COOH‐terminus. Pax4 was found to inhibit the human insulin promoter through a sequence element, the C2 box, located at −253 to −244, and the islet amyloid polypeptide promoter through a sequence element located downstream of −138. The inhibitory activity of Pax4 was mapped to separate regions of the protein between amino acids 2–230 and 231–349.