Premium
Molecular cloning, expression and immunological characterisation of Lol p 5C, a novel allergen isoform of rye grass pollen demonstrating high IgE reactivity 1
Author(s) -
Suphioglu Cenk,
Mawdsley David,
Schäppi George,
Gruehn Sabine,
de Leon Maria,
Rolland Jennifer M.,
O'Hehir Robyn E.
Publication year - 1999
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(99)01576-8
Subject(s) - immunoglobulin e , gene isoform , recombinant dna , allergen , complementary dna , molecular cloning , biology , cross reactivity , chemistry , peptide sequence , pollen , microbiology and biotechnology , antigen , allergy , antibody , biochemistry , immunology , botany , gene , cross reactions
A novel isoform of a major rye grass pollen allergen Lol p 5 was isolated from a cDNA expression library. The new isoform, Lol p 5C, shares 95% amino acid sequence identity with Lol p 5A. Both isoforms demonstrated shared antigenic activity but different allergenic activities. Recombinant Lol p 5C demonstrated 100% IgE reactivity in 22 rye grass pollen sensitive patients. In comparison, recombinant Lol p 5A showed IgE reactivity in less than 64% of the patients. Therefore, Lol p 5C represents a novel and highly IgE‐reactive isoform allergen of rye grass pollen.