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Drosophila melanogaster protein phosphatase inhibitor‐2: identification of a site important for PP1 inhibition
Author(s) -
Helps Nicholas R.,
Cohen Patricia T.W.
Publication year - 1999
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(99)01573-2
Subject(s) - drosophila melanogaster , complementary dna , phosphatase , protein phosphatase 1 , biology , microbiology and biotechnology , dusp6 , amino acid , drosophila (subgenus) , melanogaster , peptide sequence , biochemistry , protein phosphatase 2 , enzyme , gene
A database search with human protein phosphatase inhibitor‐2 (I‐2) identified a Drosophila melanogaster cDNA that encoded a protein identical in length and sharing 39% amino acid identity (58% similarity) with human I‐2. The mRNA encoding this protein is expressed in both sexes and throughout development, unlike Drosophila inhibitor‐t. The bacterially expressed protein was a specific inhibitor of protein phosphatase 1 with an IC 50 of <1 nM, confirming that it is the Drosophila homologue of mammalian inhibitor‐2. Mutation of Phe residues conserved in I‐2 from lower and higher eukaryotes showed that Phe‐33 is important for inhibition of PP1c.