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Metal regulation of metallothionein participation in redox reactions
Author(s) -
Zhang Sen,
Li Jian,
Wang Chih-Chen,
Tsou Chen-Lu
Publication year - 1999
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(99)01562-8
Subject(s) - metallothionein , dithiothreitol , chemistry , glutathione , redox , chelation , thiol , ribonuclease , zinc , reactivity (psychology) , cysteine , metal , biochemistry , enzyme , inorganic chemistry , organic chemistry , medicine , rna , alternative medicine , pathology , gene
Like glutathione or dithiothreitol, metallothionein effects the formation of pancreatic ribonuclease A from its S‐sulfonated derivative catalyzed by protein disulfide isomerase. EDTA increases the yield of ribonuclease A activity recovery with metallothionein but does not affect the reaction with glutathione or dithiothreitol. EDTA also increases the reactivity of thiol groups in metallothionein with 5,5′‐dithiobis‐(2‐nitrobenzoic acid) by chelation of zinc ions. It is suggested that the thiol groups in metallothionein form a part of the pool of cellular thiols in the regulation of cellular redox reactions and their availability is modulated by zinc chelation.