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Does diphenylene iodonium chloride have any effect on the O 2 − ‐generating step of plant peroxidases?
Author(s) -
Barceló A.Ros,
Ferrer Marı́a A.
Publication year - 1999
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(99)01542-2
Subject(s) - peroxidase , ferric , chemistry , chloride , dissociation (chemistry) , enzyme , yield (engineering) , ferric iron , biochemistry , photochemistry , inorganic chemistry , organic chemistry , thermodynamics , physics , ferrous
The O 2 − ‐generating step of plant peroxidases during their catalytic cycle is represented by the decay of compound III (CoIII) into ferriperoxidase, which most likely involves the dissociation of a ferric‐O 2 − complex to yield the ferric form of the enzyme and O 2 − . Diphenylene iodonium chloride (DPI), at 50–100 μM, does not significantly enhance the stability of CoIII of peroxidase, as judged by the values of k decay , and therefore, DPI appears to have no effect on the O 2 − ‐generating step of plant peroxidases. From these results, it is concluded that caution should be exercized when considering peroxidase as a possible enzyme target of O 2 − ‐mediated plant physiological processes sensitive to DPI inhibition.