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Intracellular localization of an active green fluorescent protein‐tagged Pho84 phosphate permease in Saccharomyces cerevisiae
Author(s) -
Petersson Jens,
Pattison Johanna,
Kruckeberg Arthur L.,
Berden Jan A.,
Persson Bengt L.
Publication year - 1999
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(99)01471-4
Subject(s) - green fluorescent protein , protein subcellular localization prediction , fusion protein , biochemistry , saccharomyces cerevisiae , transport protein , microbiology and biotechnology , membrane protein , vacuole , chemistry , biology , membrane , yeast , cytoplasm , gene , recombinant dna
Green fluorescent protein (GFP) from Aequorea victoria was used as an in vivo reporter protein when fused to the carboxy‐terminus of the Pho84 phosphate permease of Saccharomyces cerevisiae . Both components of the fusion protein displayed their native functions and revealed a cellular localization and degradation of the Pho84‐GFP chimera consistent with the behavior of the wild‐type Pho84 protein. The GFP‐tagged chimera allowed for a detection of conditions under which the Pho84 transporter is localized to its functional environment, i.e. the plasma membrane, and conditions linked to relocation of the protein to the vacuole for degradation. By use of the methodology described, GFP should be useful in studies of localization and degradation also of other membrane proteins in vivo.